Assistant Professor Tan Yong Zi: Pushing the boundaries of protein structure imaging for potential drug targets
Cryo-electron microscopy (cryo-EM) is a remarkable tool that allows scientists to view the intricate landscape of the microscopic world. It provides detailed 3D images of the molecular components of the cell, such as its DNA, RNA, and proteins. Capturing these snapshots can reveal key information about how these components interact and function in different diseases.
Using innovative cryo-EM methods, Assistant Professor Tan Yong Zi and his team from the Department of Biological Sciences under the NUS Faculty of Science constructed and revealed the protein atomic structure models that are the key to understanding the evolution of organisms, how diseases spread, and how to design targeted drugs against these diseases.
Asst Prof Tan earned a spot on this year’s TR35 Asia Pacific list as an Inventor for his cutting-edge discoveries that helped to refine cryo-EM methods for protein structure analysis.
Asst Prof Tan and his collaborators pioneered the tilted data collection strategy to capture more views of protein samples with preferred orientation problems. This strategy allowed him to solve the structures of 14 different bacterial ribosomal intermediates that can be targeted for antibiotic development. To diagnose the degree of preferred orientation present in a protein sample, Asst Prof Tan and his collaborators also developed a software, called 3DFSC, which is now an online web server widely used by structural biologists around the world.
Asst Prof Tan co-developed Back-it-up, a novel open-source cryo-EM grid freezing device that can be built at a fraction of the cost of conventional freezing device and is able to do time-resolved cryo-EM experiments. He and his collaborators also demonstrated that applying a mathematical correction called the Ewald diffraction sphere curvature correction was crucial for achieving high resolution in cryo-EM images of an adenovirus capsid sample.
“I am deeply honoured to receive this award, and I would like to acknowledge the continual support from my lab members, collaborators, mentors and NUS that has made this possible. I am also heartened to see that methods development is being recognised as an important pillar for advancing scientific progress,” said Asst Prof Tan.
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